Maternal histone messenger RNA: detection by molecular hybridization.

Identification of a novel human testicular interstitial gene, RNF148, and its expression regulated by histone deacetylases.

Multiple genes are restrictively expressed in mammalian testicular tissues, and they play important roles in the complex process of spermatogenesis. Investigation of these genes and their expression regulation mechanisms is valuable to elucidate the molecular process of spermatogenesis. In this study, we identified a novel human gene, ring finger protein 148 (RNF148) that is abundantly expresse...

Fertilization : In Vitro

Volume 2 • Issue 3 • 1000108 J Fert In Vitro ISSN: 2165-7491 JFIV, an open access journal Abbreviations: NE: Nuclear Envelope, NPC: Nuclear Pore Complex, NRN: Nuclear Routing Network, Np: Nucleoplasmin, H: histone, tRNA: Transfer Ribonucleic Acid, mRNA: Messenger Ribonucleic Acid, NLS: Nuclear Localization Signal, NES: Nuclear Export Signal, gDNA: Genomic DNA, ISH: In Situ Hybridization, scFv: ...

Visualization of single RNA transcripts in situ.

Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized with five fluorochromes per molecule, and the light emitted by a single probe was calibrated. Points of light in exhaustively deconvolved images of hybridized cells gave fluorescent intensities and distances between probe...

Origin and spatial distribution of maternal messenger RNA during oogenesis of an insect, Oncopeltus fasciatus.

In order to investigate the origin and spatial distribution of maternal mRNA during oogenesis, in situ hybridization with [3H]-poly(U) was utilized for the detection of poly(A)-containing RNA [poly(A)+RNA] in histological sections of Oncopeltus fasciatus ovaries. In the germarium poly(A)+RNA was found to accumulate in the trophocyte cytoplasm concomitant with the maturation of these cells. Poly...

Detection of specific messenger RNA by electron microscopic in situ hybridization: implications for nucleocytoplasmic transport.